Vector Database
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Vector database is a digital-only collection of vector backbone information compiled by Addgene from third party sources.
This vector is NOT available from Addgene and the database is no longer actively maintained.
This vector is not available from Addgene.
Plasmid: pHSREM3
Information
- Source/Vendor
- ATCC
- Plasmid Type
- Unspecified
- Cloning Method
- Unknown
- Size
- 3400
- Notes
- A KpnI/SacI double digest releases the hsp70 transcription module, for subsequent ligation into other plasmids such as ones containing Drosophila P elements. KpnI BglII NotI XhoI - promoter - MCS - terminator - SpeI XbaI NotI EagI BstXI SacII SacI. One of a series (ATCC 37642-37645) of plasmid vectors for expressing genes under the control of the Drosophila hsp70 promoter & terminator. The transcription module is flanked by restriction sites (facilitating its removal), T7 and T3 promoters (for in vitro RNA synthesis), & primers for Sanger sequencing. There is also an M13 origin of replication. Restriction digests of the clone give the following sizes (kb): PstI--3.5; HindIII--3.4; BamHI--3.4. (ATCC staff) The KpnI/SacI fragment of pHSREM1 (ATCC 37642) containing the hsp70 transcription module was ligated into KpnI/SacI digested Bluescript SK+. (personal communication) Major Drosophila hsp70 regulator elements are: 2 heat shock consensus elements (-85 to -72 & -62 to -49),a TATA box (-33 to -26) & transcription initiation site (+1) in the 5' segment; the termination triplet and polyA site in the 3' segment. (personal communication) Medium is 1227 LB plus ampicillin. Hosts: E.coli HB101, E.coli. (Information source: VectorDB ( http://seq.yeastgenome.org/vectordb ).)
- Catalog Number
- 37644
- Stable
- Unspecified
- Constitutive
- Unspecified
- Viral/Non-Viral
- Unspecified