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Vector Database


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Vector database is a digital-only collection of vector backbone information compiled by Addgene from third party sources.

This vector is NOT available from Addgene and the database is no longer actively maintained.

This vector is not available from Addgene.

Plasmid: pHS22

Information

Source/Vendor
ATCC
Plasmid Type
Unspecified
Cloning Method
Unknown
Size
16000
Notes
Contains hsp82-neo fusion gene which encodes the first 20 aa of D. pseudoobscura hsp82, 5 codons in a linker, fused to the fifth codon of bacterial neomycin phosphotransferase from Tn5, and followed by polyadenylation signals from D. melanogaster hsp82. [1] Genomic clone containing a complete alcohol dehydrogenase coding region from D. melanogaster (3.2 kb XbaI fragment) which includes 0.66 kb of 5' and 0.64 kb of 3' DNA, and has all the cis-acting sequences necessary for expression. [1] The order of the major components of this construct are:3' P-element, 5'-Adh-3', 5'-hsp82-neo fusion-hsp82 polyadenylation signal-3', KpnI site, and 5' P-element. [1] The hsp82 sequences begin 5 kb upstream of the transcription starting point. Expression is constitutive, but can be increased with heat. [1] Selection of transgenic Drosophila by G418 has been done with embryos, larvae, and adults. Adults can be reselected by 6% ethanol. [1] Can be used in Drosophila spp. cell culture transfection assays, and may function in other animal and plant systems because the hsp82 promoter is highly conserved. [1] P-transposable (integrating) shuttle vector. [1] Restriction digests of the clone give the following sizes (kb): KpnI--16.0; XbaI--9.4, 3.1, 0.90, 0.72, 0.56, 0.30; BamHI/KpnI--7.2, 6.6, 1.3. (ATCC staff) Medium is 1227 LB plus ampicillin. Hosts: E.coli HB101, Drosophila melanogaster, E.coli. (Information source: VectorDB ( http://seq.yeastgenome.org/vectordb ).)
Catalog Number
37840
GenBank
M32616
Stable
Unspecified
Constitutive
Unspecified
Viral/Non-Viral
Unspecified