Vector Database
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Vector database is a digital-only collection of vector backbone information compiled by Addgene from third party sources.
This vector is NOT available from Addgene and the database is no longer actively maintained.
This vector is not available from Addgene.
Plasmid: pHHluc
Information
- Source/Vendor
- ATCC
- Plasmid Type
- Unspecified
- Cloning Method
- Unknown
- Size
- 6200
- Notes
- One of a series of luciferase vectors (ATCC 37574-37584) for assaying promoter activity in mammalian cells. [1] The insert contains bp -223 to +105 of the MMTV promoter with a Restriction digests of the clone give the following sizes (kb): BamHI--6.6; HindIII--6.6; SalI--6.6; matches depositor's DNA. (ATCC staff) This is a derivative of plasmids patented by the University of California and is not to be used for commercial purposes without prior arrangement with the University of California. (personal communication) There is cryptic promoter activity which may be more readily observed with weak promoters. Be careful to do all appropriate controls and map the transcripts. (personal communication) Medium is 1227 LB plus ampicillin. Hosts: E.coli HB101, vertebrate cells, E.coli. (Information source: VectorDB ( http://seq.yeastgenome.org/vectordb ).)
- Catalog Number
- 37581
- GenBank
- M15077
- Stable
- Unspecified
- Constitutive
- Unspecified
- Viral/Non-Viral
- Unspecified