Vector Database
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Vector database is a digital-only collection of vector backbone information compiled by Addgene from third party sources.
This vector is NOT available from Addgene and the database is no longer actively maintained.
This vector is not available from Addgene.
Plasmid: pHGN3
Information
- Source/Vendor
- ATCC
- Plasmid Type
- Unspecified
- Cloning Method
- Unknown
- Size
- 4283
- Notes
- Restriction digests of the clone give the following sizes (kb): BamHI--4.2; EcoRI--4.2; SalI/HindIII--3.2, 1.0. (ATCC staff) Shuttle vector component of a virulence tester system to be used with the avirulent rabbit poxvirus deletion mutant RPmuhr23HA+ (ATCC VR-2379). [2] DNA cloned into the MCS is integrated into VR-2379 by homologous recombination, possibly altering the host range and virulence of the virus.[2] The hemagglutinin insert is a 1.8 kb 5'SalI/3'HindIII fragment containing approximately 443 bp 5' flanking sequence, 988 bp coding sequence interrupted by a polylinker, and 408 bp 3' flanking sequence. [2] The gene is interrupted at the AccI site (nt 43 of the coding region) by a 47 bp MCS from pUC9. [2] Constructed by deleting lacZ and the polylinker from pUC9 by a HaeII digest. [2] The order of the major features in the plasmid is: pUC9 delta lacZ - KpnI - 5' end of insert - PstI/MCS/EcoRI - 3' end of insert - KpnI.[2] Recombinants can be identified by inactivation of the hemagglutinin gene (HA-). [2] Medium is 1227 LB plus ampicillin. RPV = rabbit pox virus, vaccinia virus NCBI gi: 335806 Hosts: E.coli, vertebrate cells, chicken embryo fibroblasts virus-infected. (Information source: VectorDB ( http://seq.yeastgenome.org/vectordb ).)
- Catalog Number
- 69051
- GenBank
- M60387
- Stable
- Unspecified
- Constitutive
- Unspecified
- Viral/Non-Viral
- Unspecified