Vector Database
Welcome to Vector Database!
Vector database is a digital-only collection of vector backbone information compiled by Addgene from third party sources.
This vector is NOT available from Addgene and the database is no longer actively maintained.
This vector is not available from Addgene.
Plasmid: pHC79
Information
- Source/Vendor
- ATCC
- Plasmid Type
- Unspecified
- Cloning Method
- Unknown
- Size
- 6524
- Notes
- Boehringer size is 6524 bp. BRL size is 6523 bp; it is missing the c at 6481. ATCC size is 6430 bp. The lambda cohesive end site (cos region) is needed for packaging into pre-mature phage heads. pBR322 was partially digested with Sau3A and ligated with an 0.65 BglII lambda fragment, generating a BglII site (pBR322 positions 1459-1666 deleted). A 1.65 kb BglII fragment from Charon 4A was ligated into the BglII site. [1] A general purpose cosmid vector derived from pBR322. [1] Medium is 1227 LB plus ampicillin. old Boehringer vector. Hosts: E.coli HB101, E.coli LE392, E.coli JM83, E.coli 5K, E.coli. Related vectors: pBR322, lambda cos. (Information source: VectorDB ( http://seq.yeastgenome.org/vectordb ).)
- Catalog Number
- 37030
- GenBank
- L08873
- Stable
- Unspecified
- Constitutive
- Unspecified
- Viral/Non-Viral
- Unspecified