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Vector Database


Welcome to Vector Database!

Vector database is a digital-only collection of vector backbone information compiled by Addgene from third party sources.

This vector is NOT available from Addgene and the database is no longer actively maintained.

This vector is not available from Addgene.

Plasmid: pGT4.5A

Information

Source/Vendor
ATCC
Plasmid Type
Unspecified
Cloning Method
Unknown
Size
9500
Notes
Deposited by: Alexandra L. Joyner Gene trap vector designed to identify and mutate endogenous mammalian genes by integration and production of a fusion protein with the lacZ reporter gene. Can also be used to monitor endogenous gene expression. [1] Identified cDNA sequences spanning the lacZ splice junction can be cloned using the published sequence of the En-2 splice acceptor and the rapid amplification of cDNA ends (RACE) protocol. [1] Derived from pGT4.5 by replacing the En-2 polyadenylation signal with an SV40 polyadenylation signal. (personal communication) The order of the major features in this plasmid is: pUC18 - HindIII - En-2 intron - splice acceptor - En-2 homeobox exon - lacZ - SV40 polyadenylation - human beta-actin promoter - neoR - SV40 polyadenylation. [2] Restriction digests of the clone give the following sizes (kb): BamHI--3.8, 3.3, 1.8, 0.45, 0.2; HindIII--9.5; XbaI--4.6, 3.1, 1.0, 0.8. (ATCC staff) Medium is 1227 LB plus ampicillin. NCBI gi: 403021 Hosts: E.coli, E.coli HB101, vertebrate cells. (Information source: VectorDB ( http://seq.yeastgenome.org/vectordb ).)
Catalog Number
77329
GenBank
X66900, X66902, X66901, X66903
Stable
Unspecified
Constitutive
Unspecified
Viral/Non-Viral
Unspecified