Vector Database
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Vector database is a digital-only collection of vector backbone information compiled by Addgene from third party sources.
This vector is NOT available from Addgene and the database is no longer actively maintained.
This vector is not available from Addgene.
Plasmid: pGPTV-DHFR
Information
- Source/Vendor
- ATCC
- Plasmid Type
- Unspecified
- Cloning Method
- Unknown
- Size
- 13800
- Notes
- Deposited by: Robert Masterson, D. Becker Binary vector with plant selectable marker near the T-DNA left border and a glucuronidase (uidA) reporter gene. The marker and uidA are divergently transcribed, and can be easily removed or replaced. [1] The order of the major features of this plasmid is: EcoRI - pAnos - SstI - uidA - SmaI - XbaI - SalI - HindIII - Pnos - BglII - dhfr - BamHI - pAg7 - T-DNA - BglII - RK2 ori - kanR. [1] Vector contains the following restriction sites (approximate kb from the HindIII site): BamHI--1.6; EcoRI--11.6; SstI--0.9, 11.9. [1] Restriction digests of the clone give the following sizes (kb): EcoRI--13.5; BglII--8.4, 3.1, 2.3; HindIII--13.5. (ATCC staff) Medium is 1236 LB plus kanamycin. Hosts: E.coli, E.coli HB101, Agrobacterium tumefaciens, higher plants, Agrobacterium tumefaciens LBA4404. (Information source: VectorDB ( http://seq.yeastgenome.org/vectordb ).)
- Catalog Number
- 77390
- Stable
- Unspecified
- Constitutive
- Unspecified
- Viral/Non-Viral
- Unspecified