Vector Database
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Vector database is a digital-only collection of vector backbone information compiled by Addgene from third party sources.
This vector is NOT available from Addgene and the database is no longer actively maintained.
This vector is not available from Addgene.
Plasmid: pGilda
Information
- Source/Vendor
- Clontech - discontinued
- Plasmid Type
- Unspecified
- Cloning Method
- Unknown
- Size
- 6600
- Notes
- Note from Clontech: pGilda (1, 2) is a cloning vector used to generate fusions of a bait protein with the 202-residue LexA protein, which acts as a DNA-binding domain in the MATCHMAKER LexA Two-Hybrid System (#K1609-1). The hybrid protein is expressed in yeast cells from the tightly regulated GAL1 promoter (PGAL1); transcription is repressed in the presence of glucose and induced in the absence of glucose and presence of galactose (3). pGilda carries the HIS3 gene for selection in His auxotrophic yeast strains and is compatible with MATCHMAKER LexA Libraries. This vector replicates autonomously in both E. coli (using the Col E1 ori) and in S. cerevisiae (using the CEN/ARS ori). Ampr is used for selection in E. coli. Note: pGilda can also be used with other LexA two-hybrid systems, as long as the system utilizes a His-, GAL4+, GAL80+ yeast host strain having an appropriate reporter gene under the control of LexA operators.
- Catalog Number
- 6183-1
- Stable
- Unspecified
- Constitutive
- Unspecified
- Viral/Non-Viral
- Unspecified