Vector Database
Welcome to Vector Database!
Vector database is a digital-only collection of vector backbone information compiled by Addgene from third party sources.
This vector is NOT available from Addgene and the database is no longer actively maintained.
This vector is not available from Addgene.
Plasmid: pGEX-KN
Information
- Source/Vendor
- ATCC
- Plasmid Type
- Unspecified
- Cloning Method
- Unknown
- Size
- 4966
- Notes
- Deposited by: David J. Hakes, Jack E. Dixon The glutathione S-transferase (GST) fusion protein can be purified by glutathione affinity chromatography, and the desired polypeptide released from the fusion product by thrombin. [1] Expression vector for rapid purification of fusion proteins that contain no amino terminal extensions after thrombin cleavage. The amino acid after the initiator methionine must be charged. [1] Cloning into this vector requires amplification of the gene using oligonucleotides prepared as in the reference and encoding the first 4 amino acids of a thrombin recognition sequence. [1] Constructed from pGEX-1 by inserting an oligonucleotide at the BamHI site which encodes the glycine kinker and a NotI site. [1] The order of the major features in this plasmid is: pMB1 ori - lacIV - lacZ - tac promoter - GST - glycine kinker - NotI/MCS/EcoRI - ampR. [1] Restriction digests of the clone give the following sizes (kb): NotI--5.0; EcoRI/PstI--4.0, 1.0; BamHI/EcoRV--3.2, 1.8. (ATCC staff) Medium is 1227 LB plus ampicillin. Hosts: E.coli, E.coli HB101, E.coli JM101, E.coli TG1, E.coli BL21. Related vectors: pGEX-1, pGEX-KT, pGEX-KG. (Information source: VectorDB ( http://seq.yeastgenome.org/vectordb ).)
- Catalog Number
- 77332
- Stable
- Unspecified
- Constitutive
- Unspecified
- Viral/Non-Viral
- Unspecified