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Vector Database


Welcome to Vector Database!

Vector database is a digital-only collection of vector backbone information compiled by Addgene from third party sources.

This vector is NOT available from Addgene and the database is no longer actively maintained.

This vector is not available from Addgene.

Plasmid: pGEX-1

Information

Source/Vendor
ATCC
Plasmid Type
Unspecified
Cloning Method
Unknown
Size
4932
Notes
Expression vector permitting production of a fusion protein. (ATCC staff) The glutathione S-transferase (GST) fusion protein can be purified by glutathione affinity chromatography, and the desired polypeptide released from the fusion product by thrombin. [2] Constructed from pGEX-2T by inserting an oligonucleotide at the EcoRI site which encodes the glycine kinker and additional restriction sites to facilitate cloning in all reading frames. [2] The order of the major features in this plasmid are: Ptac - GST - thrombin cleavage site - BamHI site - SmaI site - glycine kinker - multiple cloning site - ampR - pBR322 ori - lacIq. [2] Restriction digests of the clone give the following sizes (kb): HindIII--5.0; BamHI--5.0; EcoRI--5.0; XbaI--5.0. (ATCC staff) Medium is 1227 LB plus ampicillin. NCBI gi: 208304 Hosts: E.coli HB101, E.coli, E.coli TG1, E.coli JM101. (Information source: VectorDB ( http://seq.yeastgenome.org/vectordb ).)
GenBank
M21676, A01437
Stable
Unspecified
Constitutive
Unspecified
Viral/Non-Viral
Unspecified

Sequence