Vector Database
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Vector database is a digital-only collection of vector backbone information compiled by Addgene from third party sources.
This vector is NOT available from Addgene and the database is no longer actively maintained.
This vector is not available from Addgene.
Plasmid: pGEM-3Zf-
Information
- Source/Vendor
- Promega
- Alt Name
- pGEM3, pGEM3Zf
- Plasmid Type
- Bacterial Expression
- Promoter
- T7 and SP6
- Expression Level
- High
- Cloning Method
- Restriction Enzyme
- Size
- 3199
- 5' Sequencing 1 Primer
- T7
- 3' Sequencing 2 Primer
- SP6
- Bacterial Resistance
- Ampicillin
- Notes
- This vector can be used for standard cloning, single-stranded DNA production and in vitro transcription from SP6 and T7 RNA polymerase promoters flanking the multiple cloning region. The sequence is the strand that is secreted by cells harboring pGEM-3Zf(-) plasmids. Hosts: E.coli JM109. Related vectors: pUC18, pGEM-3Z, pGEM-3Zf+. (Information source: <a href=http://seq.yeastgenome.org/vectordb target=_blank>VectorDB</a>.)
- GenBank
- X65307
- Stable
- Unspecified
- Constitutive
- Unspecified
- Viral/Non-Viral
- Nonviral