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Vector Database


Welcome to Vector Database!

Vector database is a digital-only collection of vector backbone information compiled by Addgene from third party sources.

This vector is NOT available from Addgene and the database is no longer actively maintained.

This vector is not available from Addgene.

Plasmid: pGEM-3Zf-

Information

Source/Vendor
Promega
Alt Name
pGEM3, pGEM3Zf
Plasmid Type
Bacterial Expression
Promoter
T7 and SP6
Expression Level
High
Cloning Method
Restriction Enzyme
Size
3199
5' Sequencing 1 Primer
T7
3' Sequencing 2 Primer
SP6
Bacterial Resistance
Ampicillin
Notes
This vector can be used for standard cloning, single-stranded DNA production and in vitro transcription from SP6 and T7 RNA polymerase promoters flanking the multiple cloning region. The sequence is the strand that is secreted by cells harboring pGEM-3Zf(-) plasmids. Hosts: E.coli JM109. Related vectors: pUC18, pGEM-3Z, pGEM-3Zf+. (Information source: <a href=http://seq.yeastgenome.org/vectordb target=_blank>VectorDB</a>.)
GenBank
X65307
Stable
Unspecified
Constitutive
Unspecified
Viral/Non-Viral
Nonviral

Sequence