Vector Database
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Vector database is a digital-only collection of vector backbone information compiled by Addgene from third party sources.
This vector is NOT available from Addgene and the database is no longer actively maintained.
This vector is not available from Addgene.
Plasmid: pFL45S
Information
- Source/Vendor
- ATCC
- Plasmid Type
- Unspecified
- Cloning Method
- Unknown
- Size
- 4063
- Notes
- pFL45S was constructed from pUC19 plasmid where two alu I sites were modified. The site 629 was replaced by a BglII linker and the site 747 by a ClaI site. The yeast selectable marker has been cloned in the BglII site and the 2 micron 0.5 Kb FspI fragment containing its replication origin has been cloned at the ClaI site. The order of the major features in this plasmid is:EcoRI/MCS/HindIII - TRP1 - 2 micron ori - pMB1 ori - ampR. YE-type high copy number shuttle vector permitting visual detection of recombinants based on alpha complementation. The loss per generation (percentage having lost all the plasmids after 20 h of non-selective growth) is 11.0. Restriction digests of the clone give the following sizes (kb): BamHI--4.2; BglII--3.4, 0.8; ClaI--3.7, 0.5. (ATCC staff) Medium is 1227 LB plus ampicillin. NCBI gi: 397133 Hosts: E.coli HB101, S.cerevisiae, E.coli, Saccharomyces. Related vectors: pUC19, yeast 2-micron plasmid. (Information source: VectorDB ( http://seq.yeastgenome.org/vectordb ).)
- Catalog Number
- 77206
- GenBank
- X70268
- Stable
- Unspecified
- Constitutive
- Unspecified
- Viral/Non-Viral
- Unspecified