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Vector Database


Welcome to Vector Database!

Vector database is a digital-only collection of vector backbone information compiled by Addgene from third party sources.

This vector is NOT available from Addgene and the database is no longer actively maintained.

This vector is not available from Addgene.

Plasmid: pFD666

Information

Source/Vendor
ATCC
Plasmid Type
Unspecified
Cloning Method
Unknown
Size
5250
Notes
The order of the major features in this plasmid is:rrnC terminator - SP6 - SphI/MCS/HindIII - T7 - trpA terminator - neo - synthetic promoter -pMB1 ori - cos - M13 ori - pJV1 ori. [1] Cosmid shuttle vector permitting production of ssDNA and containing promoters for in vitro RNA synthesis. Terminators flanking the polylinker protect the vector from transcripts originating in cloned inserts. [1] The ori of pJV1 from Streptomyces phaeochromogenes is compatible with plasmids derived from pIJ101. This vector can be used for complementation studies using multiple cloned genes in one host. [1] M13K07 helper phage improves the yield of ssDNA in E.coli with this vector. M13mp18 may also be used. [1] The neo promoter was replaced by a synthetic promoter recognized by both hosts. [1] Restriction digests of the clone give the following sizes (kb): BamHI--5.4; PstI--5.4; PvuII--3.7, 1.7; SacI--4.5, 0.9. (ATCC staff) Medium is 1236 LB plus kanamycin. Hosts: E.coli, Actinomycetes, Streptomyces lividans, Saccharopolyspora erythraea. Related vectors: pFDNEO, pJV1. (Information source: VectorDB ( http://seq.yeastgenome.org/vectordb ).)
Catalog Number
77286
Stable
Unspecified
Constitutive
Unspecified
Viral/Non-Viral
Unspecified