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Vector Database


Welcome to Vector Database!

Vector database is a digital-only collection of vector backbone information compiled by Addgene from third party sources.

This vector is NOT available from Addgene and the database is no longer actively maintained.

This vector is not available from Addgene.

Plasmid: pfd-Tn5

Information

Source/Vendor
ATCC
Plasmid Type
Unspecified
Cloning Method
Unknown
Size
8400
Notes
Deposited by: Klaus Geider Suicide plasmid for transposon-mediated or site-directed mutagenesis of Erwinia amylovora and Escherichia coli after electroporation. [1] Suggested conditions for electroporation in these bacterial strains: E. coli C600Sm, fields strength 20 kV/cm, pulse length 5 to 12 msec; Erwinia amylovora Ea7/74Sm, 25 kV/cm, 5 msec; E. amylovora Ea1/79Sm, 12.5 kV/cm, 17.5 msec. [1] To propagate the plasmid, the host must provide the bacteriophage fd gene 2 product. [1] The order of the major features in this plasmid is: EcoRI - ampR - fd ori - EcoRI - Tn5/kanR (6.5 kb). [1] Restriction digests of the clone give the following sizes (kb): EcoRI--6.5, 2.0; BamHI--4.4, 4.0; HindIII--5.0, 3.4; PstI--2.45 (doublet); 1.5, 0.96, 0.84. (ATCC staff) Medium is 1227 LB plus ampicillin. Hosts: E.coli, E.coli C600-2Sm. (Information source: VectorDB ( http://seq.yeastgenome.org/vectordb ).)
Catalog Number
77330
Stable
Unspecified
Constitutive
Unspecified
Viral/Non-Viral
Unspecified