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Vector Database


Welcome to Vector Database!

Vector database is a digital-only collection of vector backbone information compiled by Addgene from third party sources.

This vector is NOT available from Addgene and the database is no longer actively maintained.

This vector is not available from Addgene.

Plasmid: pET-49b(+)

Information

Source/Vendor
Novagen (EMD Millipore)
Plasmid Type
Bacterial Expression
Expression Level
High
Cloning Method
Unknown
Size
5900
5' Sequencing 1 Primer
T7 Fwd
5' Sequencing 1 Primer Sequence
5'd[TAATACGACTCACTATAGGG]3'
Tag 1
Trx (Nterm), His (Nterm), S-Tag (Cterm)
Bacterial Resistance
Kanamycin
Notes
Same as pET47 but also has Nterm GST Tag; contains HRV 3C Protease cleavage site for fusion tag removal at low temperatures; Cterm thrombin cleavage site
Catalog Number
71463-3
Stable
Transient
Constitutive
Constitutive
Viral/Non-Viral
Nonviral

Sequence