Vector Database
Welcome to Vector Database!
Vector database is a digital-only collection of vector backbone information compiled by Addgene from third party sources.
This vector is NOT available from Addgene and the database is no longer actively maintained.
This vector is not available from Addgene.
Plasmid: pET-21 c (+)
Information
- Source/Vendor
- EMD Biosciences
- Alt Name
- pET21c, pET-21 c, pET-21c, pET21c(+), pET-21 c(+), pET-21c(+)
- Plasmid Type
- Bacterial Expression
- Promoter
- T7
- Expression Level
- High
- Cloning Method
- Restriction Enzyme
- Size
- 5441
- 5' Sequencing 1 Primer
- T7
- 5' Sequencing 1 Primer Sequence
- 5'-TAATACGACTCACTATAGGG-3'
- 3' Sequencing 1 Primer
- T7-term
- 3' Sequencing 1 Primer Sequence
- 5'-GCTAGTTATTGCTCAGCGG-3'
- Tag 1
- T7-tag (N-term)
- Tag 2
- His-tag (C-term)
- Bacterial Resistance
- Ampicillin
- Notes
- pET-21c(+) is a bacterial expression vector that contains an N-terminal T7-Tag and an optional C-terminal His-Tag. pET-21c(+) and pET-24c(+) differ by bacterial resistance. pET-21a,b,d differ by the length of the MCS, for cloning in-frame. The f1 origin is oriented so that infection with helper phage will produce virions containing single-stranded DNA that corresponds to the coding strand.
- Catalog Number
- 69742-3
- Stable
- Transient
- Constitutive
- Constitutive
- Viral/Non-Viral
- Nonviral