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Vector Database


Welcome to Vector Database!

Vector database is a digital-only collection of vector backbone information compiled by Addgene from third party sources.

This vector is NOT available from Addgene and the database is no longer actively maintained.

This vector is not available from Addgene.

Plasmid: pEF-BOS

Information

Plasmid Type
Mammalian Expression
Cloning Method
Unknown
Size
5800
Bacterial Resistance
Ampicillin
Notes
Mizushima and Nagata, Nucleic Acids Research, 18(17)5322. SV40 ori (311 bp), human EF-1alpha promoter (1.2 kb), stuffer fragment from CDM8 vector (450 bp, cDNA to be expressed can be inserted by removing the stuffer using BstXI or XbaI), poly-A from human G-CSF (700 bp Eco81I-EcoRI fragment), into HindIII-EcoRI site of pUC119. More information: Mizushima and Nagata, Nucleic Acids Research, 18(17)5322.
Stable
Unspecified
Constitutive
Unspecified
Viral/Non-Viral
Nonviral

Sequence