Vector Database
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Vector database is a digital-only collection of vector backbone information compiled by Addgene from third party sources.
This vector is NOT available from Addgene and the database is no longer actively maintained.
This vector is not available from Addgene.
Plasmid: pED4
Information
- Plasmid Type
- Mammalian Expression
- Cloning Method
- Unknown
- Size
- 5360
- 5' Sequencing 1 Primer
- pMT2-F
- Bacterial Resistance
- Ampicillin
- Notes
- Reference: RJ Kaufman et al, Nucleic Acids Research 1991, Vol. 19, No. 16, 4485-4490. Elements: SV40, MLP, TPL, IVS, EMC-L, DHFL, SV40-pA, VA. pMT2-ECAT1 was digested with EcoRI and PstI resulting in a 2752 bp fragment. This fragment was digested with TaqI yielding an EcoRI-TaqI fragment of 508 bp. A 68-bp adapter sequence was synthesized with a 5' TaqI and a 3' XhoI end. This sequence matches the EMC virus leader sequence from nt 763-827. It also changes the ATG at position 10 within the EMC virus leader to an ATT. A three way ligation of the pMT21 EcoRI-XhoI fragment, the EMC virus EcoRI-Taq1 fragment, and the 68 bp oligonucleotide TaqI-XhoI adapter resulted in pED4.
- Stable
- Unspecified
- Constitutive
- Unspecified
- Viral/Non-Viral
- Unspecified
Published Plasmid Map
