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Vector Database


Welcome to Vector Database!

Vector database is a digital-only collection of vector backbone information compiled by Addgene from third party sources.

This vector is NOT available from Addgene and the database is no longer actively maintained.

This vector is not available from Addgene.

Plasmid: pDONR223

Information

Source/Vendor
Invitrogen
Alt Name
pDONR 223
Plasmid Type
Other, Gateway Donor vector
Cloning Method
Gateway Cloning
Size
5005
5' Sequencing 1 Primer
M13F/M13R
Bacterial Resistance
Spectinomycin
Notes
pDONR223 contains several improvements over the original Donor vector pDONR201. In pDONR223, the universal Forward (Fwd) and Reverse (Rev) sequencing primer sites flank the attP1 and attP2 recombination sites, the replication origin has been improved to increase copy number, and the selectable marker is spectinomycin resistance (instead of kanamycin resistance in pDONR201), which permits the use of either ampicillin or kanamycin resistance markers in Destination vectors. The sequences of the attP1 and attP2 sites in pDONR223 are identical to those in the original pDONR201 Entry vector. Reference: Rual et al, Genome Res. 14:2128-2135, 2004. Requires ccdB resistant strain of bacteria.
Stable
Unspecified
Constitutive
Unspecified
Viral/Non-Viral
Unspecified

Sequence