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Vector Database


Welcome to Vector Database!

Vector database is a digital-only collection of vector backbone information compiled by Addgene from third party sources.

This vector is NOT available from Addgene and the database is no longer actively maintained.

This vector is not available from Addgene.

Plasmid: pDK101

Information

Source/Vendor
ATCC
Plasmid Type
Unspecified
Cloning Method
Unknown
Size
3027
Notes
Deposited by: Bernard Weisblum Restriction digests of the clone give the following sizes (kb): BglI--1.7, 1.3; EcoRV--3.0; PstI--3.0. (ATCC staff) Vector useful for cloning-double stranded amplification products. Permits synthesis of ssDNA and contains promoters for in vitro RNA synthesis. [1] The plasmid contains the following restriction sites (bp from nt 1): BglI--1533, 2854; EcoRV--73; NcoI--37, 61; NdeI--105; NotI--85; PstI--93; PvuI--1786, 2885; PvuII--348, 2915; SacI--114; SalI--99; XcmI--44, 59; XmnI--2014. [1] The order of the major features in this plasmid is: T7/ApaI/MCS/NsiI/lacZ'SP6 - pMB1 ori - pMB1 - ampR - f1 ori. (personal communication) Medium is 1227 LB plus ampicillin. Hosts: E.coli, E.coli JM109. (Information source: VectorDB ( http://seq.yeastgenome.org/vectordb ).)
Catalog Number
77406
Stable
Unspecified
Constitutive
Unspecified
Viral/Non-Viral
Unspecified