Vector Database
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Vector database is a digital-only collection of vector backbone information compiled by Addgene from third party sources.
This vector is NOT available from Addgene and the database is no longer actively maintained.
This vector is not available from Addgene.
Plasmid: pDH5
Information
- Source/Vendor
- NCRR YRC
- Plasmid Type
- Unspecified
- Cloning Method
- Unknown
- Size
- 4726
- Notes
- Plasmid pDH5 contains the ORF for a pH-tolerant yellow-fluorescent protein (YFP), a variant of GFP. The plasmid also contains the selectable marker from S. pombe that complements S. cerevisiae his3. The codons have not been optimized for mammalian expression; instead the codons are the same as in the parent vector (except for the 5 mutations listed below). Mutations in the GFP(S65T) ORF: S65G, V68L, Q69K, S72A, T203Y. Parent vector: pFA6a-GFP(S65T)-HIS. See Wach et al. 1997.
- Stable
- Unspecified
- Constitutive
- Unspecified
- Viral/Non-Viral
- Unspecified