Vector Database
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Vector database is a digital-only collection of vector backbone information compiled by Addgene from third party sources.
This vector is NOT available from Addgene and the database is no longer actively maintained.
This vector is not available from Addgene.
Plasmid: pDH3
Information
- Source/Vendor
- NCRR YRC
- Plasmid Type
- Unspecified
- Cloning Method
- Unknown
- Size
- 4882
- Notes
- Plamid pDH3 contains the ORF for the cyan-fluorescent protein (CFP), a variant of GFP with a blue-shifted spectrum. The plasmid also contains the Kan selectable marker encoding resistance to the antibiotic G418. The codons have not been optimized for mammalian expression and instead are the same as in the parent vector (except for the 6 mutations listed below). Mutations in the GFP(S65T) ORF: F64L, Y66W, N146I, M153T, V163A, N164H. Parent vector: pFA6a-GFP(S65T)-Kan. See Wach et al. 1997.
- Stable
- Unspecified
- Constitutive
- Unspecified
- Viral/Non-Viral
- Unspecified