Vector Database
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Vector database is a digital-only collection of vector backbone information compiled by Addgene from third party sources.
This vector is NOT available from Addgene and the database is no longer actively maintained.
This vector is not available from Addgene.
Plasmid: pDG3
Information
- Source/Vendor
- ATCC
- Plasmid Type
- Unspecified
- Cloning Method
- Unknown
- Size
- 7800
- Notes
- Restriction digests of the clone give the following sizes (kb): HindIII/SalI--3.1, 1.65, 1.3, 0.75, 0.5 + smaller; XbaI--4.4, 3.4; EcoRI--7.8; SmaI--4.3, 3.5; SphI--7.8. (ATCC staff) This was constructed by inserting a HindIII/SalI fragment of Tn5 into pUC12 cut with HindIII/SalI (pNE02) and then inserting an XbaI fragment from pBB116 into the XbaI site of pNE02. [1] This is an integrative, selectable vector conferring arginine prototrophy on arginine autotrophic hosts such as Aspergillus niger 350(-)52, ATCC 20739 (argB-). It also confers kanamycin resistance.[1] Medium is 1227 LB plus ampicillin. Hosts: E.coli JM83, Aspergillus niger, E.coli, Aspergillus niger 350-52 [ATCC 20739]. (Information source: VectorDB ( http://seq.yeastgenome.org/vectordb ).)
- Catalog Number
- 53006
- Stable
- Unspecified
- Constitutive
- Unspecified
- Viral/Non-Viral
- Unspecified