Vector Database
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Vector database is a digital-only collection of vector backbone information compiled by Addgene from third party sources.
This vector is NOT available from Addgene and the database is no longer actively maintained.
This vector is not available from Addgene.
Plasmid: pCYM3-11
Information
- Source/Vendor
- ATCC
- Plasmid Type
- Unspecified
- Cloning Method
- Unknown
- Size
- 6485
- Notes
- The EcoRI, SmaI, and BamHI sites are 3' to the unique available in-phase initiation codon under control of the SV40 early promoter. Constructed by replacing the HindIII/EcoRI fragment of pCYM3 with the HindIII/EcoRI multiple cloning site from M13mp11. The translation start codon has been removed by this process. Restriction digests of the clone give the following sizes (kb): BamHI--6.3; ClaI/BamHI--3.3, 2.6, 0.5; HindIII/EcoRI--3.3, 2.0, 1.2. (ATCC staff) Shuttle expression vector. (ATCC staff) Permits expression in both S. cerevisiae and mouse cells. (ATCC staff) Medium is 1227 LB plus ampicillin. Hosts: E.coli CM5alpha, Saccharomyces cerevisiae, vertebrate cells, E.coli, Saccharomyces cerevisiae W303-1B/D, mouse C11D cell line. (Information source: VectorDB ( http://seq.yeastgenome.org/vectordb ).)
- Catalog Number
- 37829
- Stable
- Unspecified
- Constitutive
- Unspecified
- Viral/Non-Viral
- Unspecified