Vector Database
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Vector database is a digital-only collection of vector backbone information compiled by Addgene from third party sources.
This vector is NOT available from Addgene and the database is no longer actively maintained.
This vector is not available from Addgene.
Plasmid: pCYM03
Information
- Source/Vendor
- ATCC
- Plasmid Type
- Unspecified
- Cloning Method
- Unknown
- Size
- 9591
- Notes
- The EcoRI, SmaI, and BamHI sites are 3' to the unique available in-phase initiation codon under control of the SV40 early promoter. Expresses beta-galactosidase activity in E.coli, S. cerevisiae, and mouse cells, and thus can be used as a control to calibrate transformation efficency. Expression can be regulated by using medium composition to modulate copy number. Constructed by inserting a 3.048 kb EcoRI/DraI fragment from pMC1403 into pCYM3 cut with BamHI (nt 427), linkered to add an EcoRI site, and cut with BalI (nt 702). URA3d is a modified URA3 gene which is not efficiently expressed and requires a large number of plasmids to complement a ura3 mutation. Restriction digests of the clone give the following sizes (kb): BamHI/ClaI--4.9, 3.5, 0.76, 0.48; EcoRI--9.4; SmaI--9.4. (ATCC staff) Shuttle expression vector which includes a reporter group. (ATCC staff) Also permits blue/white detection in E.coli. Medium is 1227 LB plus ampicillin. Hosts: E.coli CM5alpha, Saccharomyces cerevisiae, vertebrate cells, E.coli, Saccharomyces cerevisiae W303-1B/D, mouse C11D cell line. (Information source: VectorDB ( http://seq.yeastgenome.org/vectordb ).)
- Catalog Number
- 37827
- Stable
- Unspecified
- Constitutive
- Unspecified
- Viral/Non-Viral
- Unspecified