Vector Database
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Vector database is a digital-only collection of vector backbone information compiled by Addgene from third party sources.
This vector is NOT available from Addgene and the database is no longer actively maintained.
This vector is not available from Addgene.
Plasmid: pCYM0
Information
- Source/Vendor
- ATCC
- Plasmid Type
- Unspecified
- Cloning Method
- Unknown
- Size
- 6360
- Notes
- The BamHI and EcoRI sites are 3' of the unique available in-phase initiation codon under control of the SV40 early promoter. Constructed by inserting the PvuII/BamHI fragment from pSV2-cat (SV40 promoter, small-t intron, polyadenylation signal,inactive CAT) into pUT322 (2 micron origin, URA3). Restriction digests of the clone give the following sizes (kb): BamHI--6.0; BamHI/PvuII--5.6, 0.5; EcoRI--6.0. (ATCC staff) Shuttle expression vector. (ATCC staff) Permits expression in both S. cerevisiae and mouse cells. (ATCC staff) Medium is 1227 LB plus ampicillin. Hosts: E.coli CM5alpha, Saccharomyces cerevisiae, vertebrate cells, E.coli, Saccharomyces cerevisiae W303-1B/D, mouse cell line C11D. (Information source: VectorDB ( http://seq.yeastgenome.org/vectordb ).)
- Catalog Number
- 37820
- Stable
- Unspecified
- Constitutive
- Unspecified
- Viral/Non-Viral
- Unspecified