Vector Database
Welcome to Vector Database!
Vector database is a digital-only collection of vector backbone information compiled by Addgene from third party sources.
This vector is NOT available from Addgene and the database is no longer actively maintained.
This vector is not available from Addgene.
Plasmid: pCPOT
Information
- Source/Vendor
- ATCC
- Plasmid Type
- Unspecified
- Cloning Method
- Unknown
- Size
- 11700
- Notes
- Restriction digests of the clone give the following sizes (kb): PstI--14, 4.3; AccI--3.3, 2.7, 2.7, 2.5, 2.5, 2.2, 1.5, 1.3; BglI--11.0, 4.5, 2.5; EcoRI--4.2, 3.3, 2.9, 2.9, 1.7, 1.3, 1.3. (ATCC staff) The presence of the POT1 gene ensures stable maintenance of the plasmid in yeast during growth on nonselective medium using glucose as a carbon source. [1] An episomal Saccharomyces cerevisiae/E.coli shuttle expression vector containing 2 micron circle, pBR322 replication and selection, and Schizosaccharomyces pombe sequences encoding triose phosphate isomerase (POT1). [1] Medium is 1227 LB plus ampicillin. Hosts: E.coli HB101, Saccharomyces cerevisiae, E.coli. (Information source: VectorDB ( http://seq.yeastgenome.org/vectordb ).)
- Catalog Number
- 39685
- Stable
- Unspecified
- Constitutive
- Unspecified
- Viral/Non-Viral
- Unspecified