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Vector Database


Welcome to Vector Database!

Vector database is a digital-only collection of vector backbone information compiled by Addgene from third party sources.

This vector is NOT available from Addgene and the database is no longer actively maintained.

This vector is not available from Addgene.

Plasmid: pCN60

Information

Source/Vendor
ATCC
Plasmid Type
Unspecified
Cloning Method
Unknown
Size
8700
Notes
Constructed by ligating pCN40 (ATCC 77097) and pUC18, each linearized with KpnI. Shuttle vector. (ATCC staff) Contains the basic replicon of pPS10 which has been sequenced (EMBL accession number X58896). The repA portion is flanked by EcoRI sites (nt 536 & 1500), with the site at nt 536 between the repA promoter and coding sequences. (personal communication) Retains the mobilization site of pBR322-derived vectors and can be mobilized if suitable functions are provided in trans. Restriction digests of the clone give the following sizes (kb): HindIII--8.2, 0.9; KpnI--6.0, 2.8; PstI--6.6, 2.4; XbaI--9.4; SmaI--7.6, 1.1. (ATCC staff) The order of the major features in this plasmid is:KpnI - repA - strR - PstI - SmaI - BamHI - kanR - HindIII - KpnI - MCS - HindIII - bla - pMB1 ori. Medium is 1227 LB plus ampicillin. Hosts: E.coli HB101, Pseudomonas aeruginosa, Pseudomonas putida, E.coli, Pseudomonas aeruginosa PAO1024, Pseudomonas aeruginosa PAO1162, P.putida KT2440. (Information source: VectorDB ( http://seq.yeastgenome.org/vectordb ).)
Catalog Number
77101
Stable
Unspecified
Constitutive
Unspecified
Viral/Non-Viral
Unspecified