Vector Database
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Vector database is a digital-only collection of vector backbone information compiled by Addgene from third party sources.
This vector is NOT available from Addgene and the database is no longer actively maintained.
This vector is not available from Addgene.
Plasmid: pCN38
Information
- Source/Vendor
- ATCC
- Plasmid Type
- Unspecified
- Cloning Method
- Unknown
- Size
- 4100
- Notes
- Derived from the ligation product of a 7.6 kb PstI fragment of pPS10 and a 4.2 kb PstI fragment of pKT231 which was then shortened by deletion. A 1.8 kb region of pPS10 was retained (EMBL accession number X58896) which encodes replication functions. Compatible with broad-host-range plasmids of the incompatibility groups P1, P4/Q and W, and maintained at a copy number of 15-20 in P. savastanoi. Contains the basic replicon of pPS10 which has been sequenced (EMBL accession number X58896). The repA portion is flanked by EcoRI sites (nt 536 & 1500), with the site at nt 536 between the repA promoter and coding sequences. (personal communication) General purpose vector. (ATCC staff) Restriction digests of the clone give the following sizes (kb): EcoRI--3.2, 0.95; HindIII--4.2; BamHI/KpnI--2.2, 2.0. (ATCC staff) The order of the major features in this plasmid is:PvuII - BamHI - kanR - HindIII - KpnI - repA. Medium is 1236 LB plus kanamycin. Hosts: Pseudomonas aeruginosa PAO1024 [ATCC 47053], Pseudomonas, P.aeruginosa PAO1162, P.putida KT2440 [ATCC 47054]. (Information source: VectorDB ( http://seq.yeastgenome.org/vectordb ).)
- Catalog Number
- 77095
- Stable
- Unspecified
- Constitutive
- Unspecified
- Viral/Non-Viral
- Unspecified