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Vector Database


Welcome to Vector Database!

Vector database is a digital-only collection of vector backbone information compiled by Addgene from third party sources.

This vector is NOT available from Addgene and the database is no longer actively maintained.

This vector is not available from Addgene.

Plasmid: pCMVlacZII

Information

Source/Vendor
ATCC
Plasmid Type
Unspecified
Cloning Method
Unknown
Size
10900
Notes
Shuttle reporter plasmid permitting visual detection of activity by histochemical staining. Presence of beta-galactosidase activity is used to follow cell lineage in culture or in situ. The order of the major features in this plasmid is:CMV promoter - bacterial lacZ - polyadenylation signal - RSV promoter - neoR - polyoma virus ori - SV40 ori - pMB1 ori - M13 ori. (personal communication) Restriction digests of the clone give the following sizes (kb): EcoRI--9.6, 1.3; HindIII/XbaI--7.2, 3.8; KpnI--7.4, 3.6; StuI--11.0. (ATCC staff) Medium is 1799 LB plus neomycin (10 ug/ml). Hosts: E.coli DH5alpha, vertebrate cells, E.coli. (Information source: VectorDB ( http://seq.yeastgenome.org/vectordb ).)
Catalog Number
77169
Stable
Unspecified
Constitutive
Unspecified
Viral/Non-Viral
Unspecified