Vector Database
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Vector database is a digital-only collection of vector backbone information compiled by Addgene from third party sources.
This vector is NOT available from Addgene and the database is no longer actively maintained.
This vector is not available from Addgene.
Plasmid: pCMVlacZII
Information
- Source/Vendor
- ATCC
- Plasmid Type
- Unspecified
- Cloning Method
- Unknown
- Size
- 10900
- Notes
- Shuttle reporter plasmid permitting visual detection of activity by histochemical staining. Presence of beta-galactosidase activity is used to follow cell lineage in culture or in situ. The order of the major features in this plasmid is:CMV promoter - bacterial lacZ - polyadenylation signal - RSV promoter - neoR - polyoma virus ori - SV40 ori - pMB1 ori - M13 ori. (personal communication) Restriction digests of the clone give the following sizes (kb): EcoRI--9.6, 1.3; HindIII/XbaI--7.2, 3.8; KpnI--7.4, 3.6; StuI--11.0. (ATCC staff) Medium is 1799 LB plus neomycin (10 ug/ml). Hosts: E.coli DH5alpha, vertebrate cells, E.coli. (Information source: VectorDB ( http://seq.yeastgenome.org/vectordb ).)
- Catalog Number
- 77169
- Stable
- Unspecified
- Constitutive
- Unspecified
- Viral/Non-Viral
- Unspecified