Vector Database

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Vector database is a digital-only collection of vector backbone information compiled by Addgene from third party sources.

This vector is NOT available from Addgene and the database is no longer actively maintained.

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Plasmid: pCMV-3Tag-6

Information

Source/Vendor

Stratagene

Alt Name

pCMV 3Tag 6

Plasmid Type

Mammalian Expression

Promoter

HSV TK, CMV

Expression Level

Unknown

Cloning Method

Unknown

Size

5241

5' Terminal

N-Term

Tag 1

3xFLAG

Bacterial Resistance

Ampicillin

Selectable Marker

Hygromycin

Notes

Mammalian expression vector for tagging proteins with an N-terminal 3xFLAG epitope.

Stable

Unspecified

Constitutive

Unspecified

Viral/Non-Viral

Unspecified

Sequence

Copy sequence

ATGCATTAGT TATTAATAGT AATCAATTAC GGGGTCATTA GTTCATAGCC 51 CATATATGGA GTTCCGCGTT ACATAACTTA CGGTAAATGG CCCGCCTGGC 101 TGACCGCCCA ACGACCCCCG CCCATTGACG TCAATAATGA CGTATGTTCC 151 CATAGTAACG CCAATAGGGA CTTTCCATTG ACGTCAATGG GTGGAGTATT 201 TACGGTAAAC TGCCCACTTG GCAGTACATC AAGTGTATCA TATGCCAAGT 251 ACGCCCCCTA TTGACGTCAA TGACGGTAAA TGGCCCGCCT GGCATTATGC 301 CCAGTACATG ACCTTATGGG ACTTTCCTAC TTGGCAGTAC ATCTACGTAT 351 TAGTCATCGC TATTACCATG GTGATGCGGT TTTGGCAGTA CATCAATGGG 401 CGTGGATAGC GGTTTGACTC ACGGGGATTT CCAAGTCTCC ACCCCATTGA 451 CGTCAATGGG AGTTTGTTTT GGCACCAAAA TCAACGGGAC TTTCCAAAAT 501 GTCGTAACAA CTCCGCCCCA TTGACGCAAA TGGGCGGTAG GCGTGTACGG 551 TGGGAGGTCT ATATAAGCAG AGCTGGTTTA GTGAACCGTC AGATCCGCTA 601 GCGATTACGC CAAGCTCGAA ATTAACCCTC ACTAAAGGGA ACAAAAGCTG 651 GAGCTCCACC GCGGTGGCGG CCGCCACCAT GGATTACAAG GATGACGACG 701 ATAAGGACTA TAAGGACGAT GATGACAAGG ACTACAAAGA TGATGACGAT 751 AAAGCCCGGG CGGGATCCCC CGGGCTGCAG GAATTCGATA TCAAGCTTAT 801 CGATACCGTC GACCTCGAGG GGGGGCCCGG TACCTTAATT AATTAAGGTA 851 CCAGGTAAGT GTACCCAATT CGCCCTATAG TGAGTCGTAT TACAATTCAC 901 TCGATCGGCT CGCTGATCAG CCTCGACTGT GCCTTCTAGT TGCCAGCCAT 951 CTGTTGTTTG CCCCTCCCCC GTGCCTTCCT TGACCCTGGA AGGTGCCACT 1001 CCCACTGTCC TTTCCTAATA AAATGAGGAA ATTGCATCGC ATTGTCTGAG 1051 TAGGTGTCAT TCTATTCTGG GGGGTGGGGT GGGGCAGGAC AGCAAGGGGG 1101 AGGATTGGGA AGACAATAGC AGGCATGCTG GGGAACGCGT AAATTGTAAG 1151 CGTTAATATT TTGTTAAAAT TCGCGTTAAA TTTTTGTTAA ATCAGCTCAT 1201 TTTTTAACCA ATAGGCCGAA ATCGGCAAAA TCCCTTATAA ATCAAAAGAA 1251 TAGACCGAGA TAGGGTTGAG TGTTGTTCCA GTTTGGAACA AGAGTCCACT 1301 ATTAAAGAAC GTGGACTCCA ACGTCAAAGG GCGAAAAACC GTCTATCAGG 1351 GCGATGGCCC ACTACGTGAA CCATCACCCT AATCAAGTTT TTTGGGGTCG 1401 AGGTGCCGTA AAGCACTAAA TCGGAACCCT AAAGGGAGCC CCCGATTTAG 1451 AGCTTGACGG GGAAAGCCGG CGAACGTGGC GAGAAAGGAA GGGAAGAAAG 1501 CGAAAGGAGC GGGCGCTAGG GCGCTGGCAA GTGTAGCGGT CACGCTGCGC 1551 GTAACCACCA CACCCGCCGC GCTTAATGCG CCGCTACAGG GCGCGTCAGG 1601 TGGCACTTTT CGGGGAAATG TGCGCGGAAC CCCTATTTGT TTATTTTTCT 1651 AAATACATTC AAATATGTAT CCGCTCATGA GACAATAACC CTGATAAATG 1701 CTTCAATAAT ATTGAAAAAG GAAGAGTATG AGTATTCAAC ATTTCCGTGT 1751 CGCCCTTATT CCCTTTTTTG CGGCATTTTG CCTTCCTGTT TTTGCTCACC 1801 CAGAAACGCT GGTGAAAGTA AAAGATGCTG AAGATCAGTT GGGTGCACGA 1851 GTGGGTTACA TCGAACTGGA TCTCAACAGC GGTAAGATCC TTGAGAGTTT 1901 TCGCCCCGAA GAACGTTTTC CAATGATGAG CACTTTTAAA GTTCTGCTAT 1951 GTGGCGCGGT ATTATCCCGT ATTGACGCCG GGCAAGAGCA ACTCGGTCGC 2001 CGCATACACT ATTCTCAGAA TGACTTGGTT GAGTACTCAC CAGTCACAGA 2051 AAAGCATCTT ACGGATGGCA TGACAGTAAG AGAATTATGC AGTGCTGCCA 2101 TAACCATGAG TGATAACACT GCGGCCAACT TACTTCTGAC AACGATCGGA 2151 GGACCGAAGG AGCTAACCGC TTTTTTGCAC AACATGGGGG ATCATGTAAC 2201 TCGCCTTGAT CGTTGGGAAC CGGAGCTGAA TGAAGCCATA CCAAACGACG 2251 AGCGTGACAC CACGATGCCT GTAGCAATGG CAACAACGTT GCGCAAACTA 2301 TTAACTGGCG AACTACTTAC TCTAGCTTCC CGGCAACAAT TAATAGACTG 2351 GATGGAGGCG GATAAAGTTG CAGGACCACT TCTGCGCTCG GCCCTTCCGG 2401 CTGGCTGGTT TATTGCTGAT AAATCTGGAG CCGGTGAGCG TGGGTCTCGC 2451 GGTATCATTG CAGCACTGGG GCCAGATGGT AAGCCCTCCC GTATCGTAGT 2501 TATCTACACG ACGGGGAGTC AGGCAACTAT GGATGAACGA AATAGACAGA 2551 TCGCTGAGAT AGGTGCCTCA CTGATTAAGC ATTGGCCTCA GGATTAGTGC 2601 TTCATCCCCG TGGCCCGTTG CTCGCGTTTG CTGGCGGTGT CCCCGGAAGA 2651 AATATATTTG CATGTCTTTA GTTCTATGAT GACACAAACC CCGCCCAGCG 2701 TCTTGTCATT GGCGAATTCG AACACGCAGA TGCAGTCGGG GCGGCGCGGT 2751 CCGAGGTCCA CTTCGCATAT TAAGGTGACG CGTGTGGCCT CGAACACCGA 2801 GCGACCCTGC AGCGACCCGC TTAACAGCGT CAACAGCGTG CCGCAGATCC 2851 CGGGGGGCAA TGAGATATGA AAAAGCCTGA ACTCACCGCG ACGTCTGTCG 2901 AGAAGTTTCT GATCGAAAAG TTCGACAGCG TCTCCGACCT GATGCAGCTC 2951 TCGGAGGGCG AAGAATCTCG TGCTTTCAGC TTCGATGTAG GAGGGCGTGG 3001 ATATGTCCTG CGGGTAAATA GCTGCGCCGA TGGTTTCTAC AAAGATCGTT 3051 ATGTTTATCG GCACTTTGCA TCGGCCGCGC TCCCGATTCC GGAAGTGCTT 3101 GACATTGGGG AATTCAGCGA GAGCCTGACC TATTGCATCT CCCGCCGTGC 3151 ACAGGGTGTC ACGTTGCAAG ACCTGCCTGA AACCGAACTG CCCGCTGTTC 3201 TGCAGCCGGT CGCGGAGGCC ATGGATGCGA TCGCTGCGGC CGATCTTAGC 3251 CAGACGAGCG GGTTCGGCCC ATTCGGACCG CAAGGAATCG GTCAATACAC 3301 TACATGGCGT GATTTCATAT GCGCGATTGC TGATCCCCAT GTGTATCACT 3351 GGCAAACTGT GATGGACGAC ACCGTCAGTG CGTCCGTCGC GCAGGCTCTC 3401 GATGAGCTGA TGCTTTGGGC CGAGGACTGC CCCGAAGTCC GGCACCTCGT 3451 GCACGCGGAT TTCGGCTCCA ACAATGTCCT GACGGACAAT GGCCGCATAA 3501 CAGCGGTCAT TGACTGGAGC GAGGCGATGT TCGGGGATTC CCAATACGAG 3551 GTCGCCAACA TCTTCTTCTG GAGGCCGTGG TTGGCTTGTA TGGAGCAGCA 3601 GACGCGCTAC TTCGAGCGGA GGCATCCGGA GCTTGCAGGA TCGCCGCGGC 3651 TCCGGGCGTA TATGCTCCGC ATTGGTCTTG ACCAACTCTA TCAGAGCTTG 3701 GTTGACGGCA ATTTCGATGA TGCAGCTTGG GCGCAGGGTC GATGCGACGC 3751 AATCGTCCGA TCCGGAGCCG GGACTGTCGG GCGTACACAA ATCGCCCGCA 3801 GAAGCGCGGC CGTCTGGACC GATGGCTGTG TAGAAGTACT CGCCGATAGT 3851 GGAAACCGAC GCCCCAGCAC TCGTCCGGAT CGGGAGATGG GGGAGGCTAA 3901 CTGAAACACG GAAGGAGACA ATACCGGAAG GAACCCGCGC TATGACGGCA 3951 ATAAAAAGAC AGAATAAAAC GCACGGGTGT TGGGTCGTTT GTTCATAAAC 4001 GCGGGGTTCG GTCCCAGGGC TGGCACTCTG TCGATACCCC ACCGAGACCC 4051 CATTGGGGCC AATACGCCCG CGTTTCTTCC TTTTCCCCAC CCCACCCCCC 4101 AAGTTCGGGT GAAGGCCCAG GGCTCGCAGC CAACGTCGGG GCGGCAGGCC 4151 CTGCCATAGC CACTGGCCCC GTGGGTTAGG GACGGGGTCC CCCATGGGGA 4201 ATGGTTTATG GTTCGTGGGG GTTATTATTT TGGGCGTTGC GTGGGGTCAG 4251 GTCCACGACT GGACTGAGCA GACAGACCCA TGGTTTTTGG ATGGCCTGGG 4301 CATGGACCGC ATGTACTGGC GCGACACGAA CACCGGGCGT CTGTGGCTGC 4351 CAAACACCCC CGACCCCCAA AAACCACCGC GCGGATTTCT GGCGCCGCCG 4401 GACGAACTAA ACCTGACTAC GACATCTCTG CCCCTTCTTC GCTGGTACGA 4451 GGAGCGCTTT GCCTCAGGTC ATGACCAAAA TCCCTTAACG TGAGTTTTCG 4501 TTCCACTGAG CGTCAGACCC CGTAGAAAAG ATCAAAGGAT CTTCTTGAGA 4551 TCCTTTTTTT CTGCGCGTAA TCTGCTGCTT GCAAACAAAA AAACCACCGC 4601 TACCAGCGGT GGTTTGTTTG CCGGATCAAG AGCTACCAAC TCTTTTTCCG 4651 AAGGTAACTG GCTTCAGCAG AGCGCAGATA CCAAATACTG TTCTTCTAGT 4701 GTAGCCGTAG TTAGGCCACC ACTTCAAGAA CTCTGTAGCA CCGCCTACAT 4751 ACCTCGCTCT GCTAATCCTG TTACCAGTGG CTGCTGCCAG TGGCGATAAG 4801 TCGTGTCTTA CCGGGTTGGA CTCAAGACGA TAGTTACCGG ATAAGGCGCA 4851 GCGGTCGGGC TGAACGGGGG GTTCGTGCAC ACAGCCCAGC TTGGAGCGAA 4901 CGACCTACAC CGAACTGAGA TACCTACAGC GTGAGCTATG AGAAAGCGCC 4951 ACGCTTCCCG AAGGGAGAAA GGCGGACAGG TATCCGGTAA GCGGCAGGGT 5001 CGGAACAGGA GAGCGCACGA GGGAGCTTCC AGGGGGAAAC GCCTGGTATC 5051 TTTATAGTCC TGTCGGGTTT CGCCACCTCT GACTTGAGCG TCGATTTTTG 5101 TGATGCTCGT CAGGGGGGCG GAGCCTATGG AAAAACGCCA GCAACGCGGC 5151 CTTTTTACGG TTCCTGGCCT TTTGCTGGCC TTTTGCTCAC ATGTTCTTTC 5201 CTGCGTTATC CCCTGATTCT GTGGATAACC GTATTACCGC C