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Vector Database


Welcome to Vector Database!

Vector database is a digital-only collection of vector backbone information compiled by Addgene from third party sources.

This vector is NOT available from Addgene and the database is no longer actively maintained.

This vector is not available from Addgene.

Plasmid: pCE54

Information

Source/Vendor
ATCC
Plasmid Type
Unspecified
Cloning Method
Unknown
Size
14500
Notes
This was constructed by ligating pCG2 (6.6 kb) linearized with PstI to pGA22 (7.9 kb) linearized with PstI. [1] This vector contains the following restriction sites (in approximate kb from one PstI site): KpnI--0.8, 3.8; BamHI--4.4, 10.7, 13.4; HpaI--5.8; EcoRI--8.3; SalI--10.3; XhoI--12.5. [1] The pGA22 portion of the construct contains the drug resistance genes. cmlR can be insertionally inactivated by cloning into the EcoRI site; kanR can be inactivated by cloning into the HindIII or XhoI sites. [2] The original vials from the depositor are being used for distribution. (ATCC staff) Growth at 30C in the presence of chloramphenicol, kanamycin, or tetracycline was verified. Recommended tetracycline concentration is 1.5 ug/ml on LB agar. (ATCC staff) Medium is -1 LB plus tetracycline (1.5 ug/ml). Hosts: Corynebacterium glutamicum LA103, Corynebacterium glutamicum, Brevibacterium, E.coli. (Information source: VectorDB ( http://seq.yeastgenome.org/vectordb ).)
Catalog Number
39019
Stable
Unspecified
Constitutive
Unspecified
Viral/Non-Viral
Unspecified