Vector Database
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Vector database is a digital-only collection of vector backbone information compiled by Addgene from third party sources.
This vector is NOT available from Addgene and the database is no longer actively maintained.
This vector is not available from Addgene.
Plasmid: pcDL-SRa296
Information
- Alt Name
- pcDL-SRalpha296
- Plasmid Type
- Mammalian Expression
- Promoter
- SRalpha
- Cloning Method
- Unknown
- Size
- 3700
- Notes
- See Takebe et al, 1988, MCB 8: 466-472. This vector is composed of (i) the plasmid linker (HindIII-PstI) segment (802 bp) which provides the SRa promoter and SV40 late-gene splicing junction; (ii) the vector-primer (KpnI-HindIII) segment (2,606 bp) which comprises the KpnI-oligo(dT) priming site, SV40 late-region polyadenylation signal [poly(A)], and pUC plasmid-derived vector sequence (the pBR322 replication origin and beta-lactamase [Ap^R] gene lacking a PstI site [deltaPstI] are shown; the NdeI site in the vector sequence of this particular construct is converted to a SalI site for the convenience of excision of an entire cDNA transcriptional unit by SalI cleavage); and (iii) the intervening DNA piece (PstI-KpnI) (336 bp) between the plasmid linker and vector-primer segments, consisting of the SV40 DNA fragment flanked by the EcoRI (SV40 map position 0.754) and KpnI (map position 0.715) sites from plasmid pcDV1 and the short DNA (PstI-EcoRI) fragment from plasmid pLl. More information: See Takebe et al, 1988, MCB 8: 466-472.
- Stable
- Unspecified
- Constitutive
- Unspecified
- Viral/Non-Viral
- Unspecified
Published Plasmid Map
