Vector Database
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Vector database is a digital-only collection of vector backbone information compiled by Addgene from third party sources.
This vector is NOT available from Addgene and the database is no longer actively maintained.
This vector is not available from Addgene.
Plasmid: pC4ro3
Information
- Source/Vendor
- ATCC
- Plasmid Type
- Unspecified
- Cloning Method
- Unknown
- Size
- 10800
- Notes
- Vector containing a non-autonomous P-element and incorporating the rough gene (ro) as a dominant marker for P-element mediated gene transfer into the germ line of Drosophila melanogaster. The order of the major features in this plasmid is: HindIII - P-element/Carnegie 4/P-element - XhoI/MCS/SphI - ro gene. The SphI/HindIII fragment corresponding to the rough gene contains the following restriction sites (kb from the 5' end): BamHI--3.02; BglII--5.39; EcoRI--0.91, 4.91; SacI--0.57, 4.19, 5.86; SalI--1.80, 3.91; SmaI--4.43. (personal communication) Restriction digests of the clone give the following sizes (kb): SalI--8.6, 2.2; HindIII--7.7, 3.1; PstI--10.8. (ATCC staff) Medium is 1227 LB plus ampicillin. Hosts: E.coli HB101, Drosophila melanogaster, E.coli, E.coli TG1, E.coli RR1. (Information source: VectorDB ( http://seq.yeastgenome.org/vectordb ).)
- Catalog Number
- 77319
- Stable
- Unspecified
- Constitutive
- Unspecified
- Viral/Non-Viral
- Unspecified