Vector Database
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Vector database is a digital-only collection of vector backbone information compiled by Addgene from third party sources.
This vector is NOT available from Addgene and the database is no longer actively maintained.
This vector is not available from Addgene.
Plasmid: pBS10
Information
- Source/Vendor
- NCRR YRC
- Plasmid Type
- Unspecified
- Cloning Method
- Unknown
- Size
- 4875
- Notes
- Plasmid pBS10 is based upon pBS4, where CFP has been mutated to the Cerulean variant. Those mutations are described in An Improved Cyan Fluorescent Protein Variant Useful for FRET by Rizzo, Springer, Granada, and Piston in Nat Biotechnol 2004;22:445-9. In addition to the mutations described we also needed to reverted the N164H and L231H found in our CFP but not the ECFP they used. Some common restriction enzyme sites are shown. Unique sites are in red. The forward and reverse primers are the same as in pDH3.
- Stable
- Unspecified
- Constitutive
- Unspecified
- Viral/Non-Viral
- Unspecified