Vector Database
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Vector database is a digital-only collection of vector backbone information compiled by Addgene from third party sources.
This vector is NOT available from Addgene and the database is no longer actively maintained.
This vector is not available from Addgene.
Plasmid: pBLCAT6
Information
- Source/Vendor
- ATCC
- Plasmid Type
- Unspecified
- Cloning Method
- Unknown
- Size
- 4256
- Notes
- Promoter-cloning vector for analysis of promoters in vertebrate cells, using the CAT reporter gene. Constructed from pBLCAT3 (ATCC37528) by deletion of a 467 bp fragment immediately upstream from the 5' polylinker and replacement by two fragments, each containing the polyadenylation signal of the SV40 large T gene. The vector contains the following restriction sites (approximate kb from nt 1): BglI--3.07; ClaI--1.69; EcoRI--0.30, 1.71; HindIII--0.0; NcoI--0.60; PvuI--3.33; PvuII--0.20, 1.89; SacI--1.70; XhoI--0.05. The order of the major features in the plasmid is: two SV40 polyadenylation signals - HindIII/MCS/XhoI - cat - SV40 small T splice site - SV40 polyadenylation signal - ClaI/MCS/SstI - pMB1 ori - ampR. [1] Deposited by: M.D.Boshart Growth media: LB plus ampicillin (ATCC medium number 1227) 37C Hosts: E.coli. (Information source: VectorDB ( http://seq.yeastgenome.org/vectordb ).)
- Catalog Number
- 77413
- GenBank
- M80484
- Stable
- Unspecified
- Constitutive
- Unspecified
- Viral/Non-Viral
- Unspecified