Vector Database
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Vector database is a digital-only collection of vector backbone information compiled by Addgene from third party sources.
This vector is NOT available from Addgene and the database is no longer actively maintained.
This vector is not available from Addgene.
Plasmid: pBJ1-Neo
Information
- Alt Name
- pBJ1
- Plasmid Type
- Mammalian Expression
- Promoter
- SR alpha
- Cloning Method
- Unknown
- Size
- 6600
- 5' Sequencing 1 Primer
- SV40pro-F
- Bacterial Resistance
- Ampicillin
- Selectable Marker
- Neomycin
- Notes
- From Mark Davis lab: Lin et. al. Science 1990, 249 (4969) 677-679. pBJ1-Neo is a derivative of pcDL-SR alpha296 [Y. Takebe et al., MCB 8, 466 (1988)] in which an Xho I and Xho I/Bam HI fragment located between the SR alpha (human T cell lymphotropic virus type 1) promoter and the SV40 polyadenylation site has been replaced for convenient cDNA cloning by a polylinker that contains the following restriction sites: 5'-Xho I-Xba I-Sfi I-Not I- Eco RI-Eco RV- Hind III-Cla I-3'. In addition, a neomycin gene (which confers resistance to the antibiotic G418) was inserted in between the ampicillin resistance gene and the SR alpha promoter at the Pvu I site. More information: From Mark Davis lab: Lin et. al. Science 1990, 249 (4969) 677-679.
- Stable
- Unspecified
- Constitutive
- Unspecified
- Viral/Non-Viral
- Unspecified