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Vector Database


Welcome to Vector Database!

Vector database is a digital-only collection of vector backbone information compiled by Addgene from third party sources.

This vector is NOT available from Addgene and the database is no longer actively maintained.

This vector is not available from Addgene.

Plasmid: pBIIHI.2

Information

Source/Vendor
ATCC
Plasmid Type
Unspecified
Cloning Method
Unknown
Size
5200
Notes
Permits positive selection for inserts, based on the dominance of the wild-type rpsL (ribosomal protein S12) gene in the plasmid. The SmaI and one HpaI site are in this gene. (ATCC staff) Derived from pNO1523 by inserting a BglII linker at the EcoRI site and a HpaI linker at the PvuII site. (personal communication) The order of the major features in this plasmid is:EcoRI - BglII - EcoRI - ClaI - HindIII - BamHI - rpsL - ori - bla. (personal communication) Restriction digests of the clone give the following sizes (kb): EcoRI--5.2; BglII--5.2; HindIII--5.2; PstI--3.6, 1.6. (ATCC staff) Medium is 1315 1065 plus ampicillin (100 ug/ul). Hosts: E.coli RR1, E.coli. (Information source: VectorDB ( http://seq.yeastgenome.org/vectordb ).)
Catalog Number
67902
Stable
Unspecified
Constitutive
Unspecified
Viral/Non-Viral
Unspecified