Vector Database
Welcome to Vector Database!
Vector database is a digital-only collection of vector backbone information compiled by Addgene from third party sources.
This vector is NOT available from Addgene and the database is no longer actively maintained.
This vector is not available from Addgene.
Plasmid: pAS2
Information
- Source/Vendor
- ATCC
- Plasmid Type
- Unspecified
- Cloning Method
- Unknown
- Size
- 8500
- Notes
- Restriction digests of the clone give the following sizes (kb): BamHI--8.6; SalI--8.6; SmaI--8.6. (ATCC staff) Shuttle expression vector used to create fusion proteins consisting of the nuclear localization sequence from SV40 T antigen, the GAL4 DNA-binding domain (aa 1-147), and a HA (hemagglutinin) epitope tag in frame with the activation domain. [1] The order of the major features in this plasmid is: ADC1 (ADH) promoter -> - GAL4 DNA binding domain - HA - NdeI/MCS/SalI - ADC1 terminator - pMB1 ori - ampR - 2 micron ori - TRP1 -> - f1 ori - <- CYH2. [2] Growth: LB plus ampicillin (ATCC medium number 1227) 37C Deposited by: Elledge S.J. Hosts: E.coli, yeast, Saccharomyces cerevisiae. (Information source: VectorDB ( http://seq.yeastgenome.org/vectordb ).)
- Catalog Number
- 87008
- Stable
- Unspecified
- Constitutive
- Unspecified
- Viral/Non-Viral
- Unspecified