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Vector Database


Welcome to Vector Database!

Vector database is a digital-only collection of vector backbone information compiled by Addgene from third party sources.

This vector is NOT available from Addgene and the database is no longer actively maintained.

This vector is not available from Addgene.

Plasmid: pAB520

Information

Source/Vendor
ATCC
Plasmid Type
Unspecified
Cloning Method
Unknown
Size
4100
Notes
That expression was improved by modifying the 17 bp before the initiation codon in pAB366 to match the sequence of the am gene. Shuttle vector conferring bleomycin and phleomycin resistance. Expression of ble (bleomycin resistance) is regulated by the promoter and terminator regions of the am gene (amination, glutamate dehydrogenase) from N. crassa. The am::ble fusion cassette can be removed by digestion with either HindIII, SphI, XbaI or BamHI or any combination of them. The order of the major features in this plasmid is:pUC18 - MCS (HindIII to BamHI) - am promoter - ble - XbaI - am 3' region - EcoRI - MCS (HindIII to EcoRI) - pUC18. Restriction digests of the clone give the following sizes (kb): HindIII--2.7, 1.4; KpnI--4.1; SmaI--3.1, 1.0. (ATCC staff) A (GT)n repeat. (personal communication) Medium is 1227 LB plus ampicillin. Hosts: E.coli HB101, Aspergillus nidulans, Neurospora crassa, E.coli. (Information source: VectorDB ( http://seq.yeastgenome.org/vectordb ).)
Catalog Number
77137
Stable
Unspecified
Constitutive
Unspecified
Viral/Non-Viral
Unspecified