Skip to main content
Addgene

Vector Database


Welcome to Vector Database!

Vector database is a digital-only collection of vector backbone information compiled by Addgene from third party sources.

This vector is NOT available from Addgene and the database is no longer actively maintained.

This vector is not available from Addgene.

Plasmid: pAB366Ben

Information

Source/Vendor
ATCC
Plasmid Type
Unspecified
Cloning Method
Unknown
Size
7200
Notes
Shuttle reporter plasmid to test expression of bleomycin resistance in transformants. [1] Expresses the mutant beta-tubulin gene conferring benomyl resistance. [1] A 2.5 kb SalI/HindIII fragment contains the complete tubulin gene and 5' and 3' flanking regions. [2] The order of the major features in this plasmid is:pUC8 - EcoRI - BamHI - am promoter - ble - XhoI - am 3' region - tub-2 (benomyl resistance) - pUC8. [1] Restriction digests of the clone give the following sizes (kb): BamHI--5.1, 2.1; HindIII--4.0, 3.2; BamHI/HindIII--2.6, 2.5, 1.5, 0.65; BamHI/XhoI--3.0, 1.35, 1.2, 0.9, 0.8; SalI/HindIII--3.3, 2.6, 0.85, 0.55. (ATCC staff) A (GT)n repeat. (personal communication) Medium is 1227 LB plus ampicillin. Hosts: E.coli HB101, Aspergillus nidulans, Neurospora crassa, E.coli. (Information source: VectorDB ( http://seq.yeastgenome.org/vectordb ).)
Catalog Number
77136
GenBank
M13630
Stable
Unspecified
Constitutive
Unspecified
Viral/Non-Viral
Unspecified