Vector Database
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Vector database is a digital-only collection of vector backbone information compiled by Addgene from third party sources.
This vector is NOT available from Addgene and the database is no longer actively maintained.
This vector is not available from Addgene.
Plasmid: p-delta-blue
Information
- Source/Vendor
- ATCC
- Plasmid Type
- Unspecified
- Cloning Method
- Unknown
- Size
- 3507
- Notes
- The insert to be cloned should have a phosphorylated 5' blunt end with the first base corresponding to the first base of the first codon. (personal communication) Expression vector producing proteins with the one additional N-terminal methionine. A modified lacZ' allows blue/white screening of colonies.[1] Constructed from pfXblue (ATCC 77333) by deletion of lambda cII and coagulation factor Xa protease recognition sequences (nt 824-943). [1] Inserts can be cloned into the vector using a filled-in NcoI site and a MCS restriction site matching the 3' end of the insert. The vector supplies the AUG initiator codon. (personal communication) The order of the major features of the plasmid is: pMB1 ori - ampR - lambdaPL promoter - NcoI/MCS/HindIII/modified lacZ'. [1] Restriction digests of the plasmid give the following sizes (kb): BamHI--3.507; BglI--1.784, 1.723; PstI--2.072, 1.435; PvuI--2.063, 1.444; PvuII--2.852, 0.655; SacI--3.257, 0.25. (personal communication) Restriction digests of the clone give the following sizes (kb): BglI--1.75 (doublet); EcoRI--3.5; PstI--2.1, 1.45. (ATCC staff) Medium is 1227 LB plus ampicillin. NCBI gi: 208983 Hosts: E.coli, E.coli HB101. Related vectors: pfXblue. (Information source: VectorDB ( http://seq.yeastgenome.org/vectordb ).)
- Catalog Number
- 77334
- GenBank
- M88655
- Stable
- Unspecified
- Constitutive
- Unspecified
- Viral/Non-Viral
- Unspecified