Vector Database
Welcome to Vector Database!
Vector database is a digital-only collection of vector backbone information compiled by Addgene from third party sources.
This vector is NOT available from Addgene and the database is no longer actively maintained.
This vector is not available from Addgene.
Plasmid: Mtchi5
Information
- Source/Vendor
- ATCC
- Plasmid Type
- Unspecified
- Cloning Method
- Unknown
- Size
- 10700
- Notes
- This vector contains the following sites separated by (bp): Sau3A I - 130 - BamHI - 280 - SalI - 3700 - EcoRI - 2230 - EcoRI - 860 - SalI - 3250 - HindIII - 255 - Sau3A I. A 370 bp HaeIII/AluI fragment encoding dihydrofolate reductase (ends filled, BamHI & HindIII linkers added) was inserted between the BamHI and HindIII sites of YEp13. DHFR transcription runs from BamHI toward the HindIII site. An episomal Saccharomyces cerevisiae/E.coli shuttle vector. Restriction digests of the clone give the following sizes (kb): BamHI--11.0; HindIII--11.0; EcoRI--11.5. (ATCC staff) Medium is 1227 LB plus ampicillin. Hosts: E.coli LE392, Saccharomyces cerevisiae, E.coli. (Information source: VectorDB ( http://seq.yeastgenome.org/vectordb ).)
- Catalog Number
- 39275
- Stable
- Unspecified
- Constitutive
- Unspecified
- Viral/Non-Viral
- Unspecified