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Vector Database


Welcome to Vector Database!

Vector database is a digital-only collection of vector backbone information compiled by Addgene from third party sources.

This vector is NOT available from Addgene and the database is no longer actively maintained.

This vector is not available from Addgene.

Plasmid: Mtchi4

Information

Source/Vendor
ATCC
Plasmid Type
Unspecified
Cloning Method
Unknown
Size
10700
Notes
This vector contains the following sites separated by (bp): Sau3A I - 255 - BamHI - 280 - SalI - 3700 - EcoRI - 2230 - EcoRI - 860 - SalI - 3250 - HindIII - 130 - Sau3A I. DHRF transcription runs from HindIII toward the BamHI site. This plasmid does NOT confer methotrexate resistance in yeast but does confer trimethoprim resistance in E.coli. A 370 bp HaeIII/AluI fragment encoding dihydrofolate reductase (ends filled, BamHI & HindIII linkers added) was inserted between the BamHI and HindIII sites of YEp13. An episomal Saccharomyces cerevisiae/E.coli shuttle vector. Restriction digests of the clone give the following sizes (kb): HindIII--10.5; BamHI--10.5; PstI--5.8, 4.4. (ATCC staff) Medium is 1227 LB plus ampicillin. Hosts: E.coli LE392, Saccharomyces cerevisiae, E.coli. (Information source: VectorDB ( http://seq.yeastgenome.org/vectordb ).)
Catalog Number
39276
Stable
Unspecified
Constitutive
Unspecified
Viral/Non-Viral
Unspecified