Vector Database
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Vector database is a digital-only collection of vector backbone information compiled by Addgene from third party sources.
This vector is NOT available from Addgene and the database is no longer actively maintained.
This vector is not available from Addgene.
Plasmid: LZRSpBMN-Z
Information
- Plasmid Type
- Unspecified
- Cloning Method
- Unknown
- Bacterial Resistance
- Ampicillin
- Selectable Marker
- Puromycin
- Notes
- Kinsella, T. M., and Nolan, G. P. Episomal vectors rapidly and stably produce high-titer recombinant retrovirus. Hum. Gene Ther., 7: 1405-1413, 1996. This plasmid contains the full length Moloney LTRs and the extended phi sequence and carries lacZ cDNA under the transcriptional control of the 5' LTR. Outside the LTRs, it contains the PGK-l promoter that drives puromycin resistance, the EBV origin of replication, and the EBNA-l gene. The last two endow the plasmid with the capacity to replicate as an episome and develop 5-20copies/mammalian cell. More information: Kinsella, T. M., and Nolan, G. P. Episomal vectors rapidly and stably produce high-titer recombinant retrovirus. Hum. Gene Ther., 7: 1405-1413, 1996.
- Stable
- Unspecified
- Constitutive
- Unspecified
- Viral/Non-Viral
- Unspecified