Vector Database
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Vector database is a digital-only collection of vector backbone information compiled by Addgene from third party sources.
This vector is NOT available from Addgene and the database is no longer actively maintained.
This vector is not available from Addgene.
Plasmid: lambdaSK22
Information
- Source/Vendor
- ATCC
- Plasmid Type
- Unspecified
- Cloning Method
- Unknown
- Size
- 47800
- Notes
- Restriction digests of the clone give the following sizes (kb): BamHI--25.0, 13.5, 9.5; EcoRI--25.0, 13.5, 9.5; SalI--20.0, 9.5, 9.2, 4.2, 3.9. (ATCC staff) Vector permitting positive selection (Spi) of recombinants. Useful for generating jumping and linking libraries and conversion to phagemid (without selection). Permits construction using partial filling-in. The sizes of the major components are (kb): arms--20.3, 9.2; stuffer fragment--13.7; phagemid segments--3.9 (longer arm), 0.7. The polylinker sites given are those associated with the longer arm. The sites associated with the other arm are: SalI BamHI EcoRI XhoI NotI NaeI SfiI AvrII NheI NcoI XbaI. The restriction sites to be used for conversion to phagemid are: ClaI BspMII SalI. The order of the major features in this plasmid is: arm - ClaI BspMII SalI - pMB1 ori - ampR - M13 ori - SacI/MCS/SalI - stuffer - SalI/MCS/XbaI - ClaI SalI - arm. [2] Deposited by: Zabarovsky E.R., Winberg G.R., Klein G.R. Hosts: E.coli. (Information source: VectorDB ( http://seq.yeastgenome.org/vectordb ).)
- Catalog Number
- 86972
- Stable
- Unspecified
- Constitutive
- Unspecified
- Viral/Non-Viral
- Unspecified