Vector Database
Welcome to Vector Database!
Vector database is a digital-only collection of vector backbone information compiled by Addgene from third party sources.
This vector is NOT available from Addgene and the database is no longer actively maintained.
This vector is not available from Addgene.
Plasmid: lambdaSK20
Information
- Source/Vendor
- ATCC
- Plasmid Type
- Unspecified
- Cloning Method
- Unknown
- Size
- 47800
- Notes
- Restriction digests of the clone give the following sizes (kb): BamHI--21.0, 13.5 (doublet); EcoRI--21.0, 13.5 (doublet); SalI--20.3, 12.0, 9.4, 6.0. (ATCC staff) Vector permitting positive selection (Spi) of recombinants. Useful for conversion to phagemid (without selection). Permits construction using partial filling-in. The sizes of the major components are (kb): arms--20.3, 9.2; stuffer fragment--13.7; phagemid segments--0.7 (longer arm), 3.9. The restriction sites to be used for conversion to phagemid are: SalI SacII BglI SfiI NotI XmaIII. Cloning capacity: 18.9 kb. The order of the major features in this plasmid is: arm - SalI/XmaIII - XbaI/MCS/NaeI - stuffer - NaeI/MCS/SacI - M13 ori - ampR - pMB1 ori - XmaIII/SalI - arm. [2] Deposited by: Zabarovsky E.R., Winberg G.R., Klein G.R. Hosts: E.coli. (Information source: VectorDB ( http://seq.yeastgenome.org/vectordb ).)
- Catalog Number
- 86970
- Stable
- Unspecified
- Constitutive
- Unspecified
- Viral/Non-Viral
- Unspecified