Vector Database
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Vector database is a digital-only collection of vector backbone information compiled by Addgene from third party sources.
This vector is NOT available from Addgene and the database is no longer actively maintained.
This vector is not available from Addgene.
Plasmid: lambda N- cI857 r32
Information
- Source/Vendor
- ATCC
- Plasmid Type
- Unspecified
- Cloning Method
- Unknown
- Notes
- This was isolated as spontaneous mutant of lambda papa prophage. It contains an IS2 terminator between the tR1 site and the O gene. This replicates in sup hosts (clear plaques at 42C; turbid plaques at 30C). It does not grow on sup0 hosts and does not kill a sup0 host when lysogenized and induced at 42C. (personal communication) This is bacteriophage lambda with mutant O and P genes regulated by the N product (functional only when N is supplied). It produces immunity to lambda. Genotype:Nam7 Nam53 cI857 r32 IS2 Medium is 1592 SM buffer. Hosts: bacteria-free lysate, E.coli, E.coli C600. (Information source: VectorDB ( http://seq.yeastgenome.org/vectordb ).)
- Catalog Number
- 40120
- Stable
- Unspecified
- Constitutive
- Unspecified
- Viral/Non-Viral
- Unspecified