Vector Database
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Vector database is a digital-only collection of vector backbone information compiled by Addgene from third party sources.
This vector is NOT available from Addgene and the database is no longer actively maintained.
This vector is not available from Addgene.
Plasmid: EMSV
Information
- Alt Name
- pEMSVscribe
- Plasmid Type
- Mammalian Expression
- Cloning Method
- Unknown
- Size
- 5300
- Bacterial Resistance
- Ampicillin
- Selectable Marker
- Puromycin
- Notes
- Davis RL, Weintraub H, Lassar AB., Cell. 1987 Dec 24;51(6):987-1000. pEMSVscribe was generated by first digesting Bluescribe M13(+) (Stratagene) with EcoRI, filling in the ends with the Klenow fragment of E. coli pol I (Boehringer Mannheim), and religating to create Bluescribe M13(+)EcoRI-. This vector was then cut with Hindlll, and the Hindlll fragment of EMSV-33 (Harland and Weintraub, 1985) containing the Moloney sarcoma virus LTR, and SV40 poly(A) addition signal was inserted by religation. This construct allows unique EcoRl insertion between the LTR and poly(A) signal sequence, which are themselves flanked by T7 and T3 promoters. More information: Davis RL, Weintraub H, Lassar AB., Cell. 1987 Dec 24;51(6):987-1000.
- Stable
- Unspecified
- Constitutive
- Unspecified
- Viral/Non-Viral
- Unspecified