Vector Database
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Vector database is a digital-only collection of vector backbone information compiled by Addgene from third party sources.
This vector is NOT available from Addgene and the database is no longer actively maintained.
This vector is not available from Addgene.
Plasmid: BSB-
Information
- Source/Vendor
- ATCC
- Plasmid Type
- Unspecified
- Cloning Method
- Unknown
- Size
- 3210
- Notes
- Created by Moore, July 1995, under contract with NCBI. Constructed from Bluescribe M13- by insertion of a synthetic BstXI adapter between the PstI & XbaI sites of the polylinker region. A plasmid vector containing convenient priming sites for sequence analysis and synthesis of RNA in either direction from T3 and T7 promoters. BSB+ (away from cloning site) and BSB- (toward cloning site) differ in their orientation of the intergenic region from bacteriophage f1. Contains the intergenic region from bacteriophage f1 permitting synthesis of single-strand copies of the insert. Restriction digests of the clone give the following sizes (kb): NdeI/HindIII--2.45, 0.75; BamHI--3.2; NdeI--3.2; PvuII--2.65 + smaller; PstI--uncut. (ATCC staff) Medium is 1227 LB plus ampicillin. Hosts: E.coli HB101, E.coli. Related vectors: BlueScribe M13-. (Information source: VectorDB ( http://seq.yeastgenome.org/vectordb ).)
- Catalog Number
- 67725
- Stable
- Unspecified
- Constitutive
- Unspecified
- Viral/Non-Viral
- Unspecified